Review



vegf a  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Miltenyi Biotec vegf a
    Vegf A, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vegf/Human+VEGF+(165)+IS%2C+premium+grade/pmc13380759-111-13-17
    Average 94 stars, based on 26 article reviews
    vegf a - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    FACS:

    Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19.
    Article Snippet: The expanded ECs were frozen in 40% EGM-2 (Promocell), 10% dimethyl sulfoxide and 50% fetal calf serum (v/v) to be thawed upon use. .. Differentiated endothelial cells have previously been extensively characterized using FACS, immunofluorescence and RNA sequencing, and have a distinct phenotype that mostly resembles an embryonic state, but also shows signs of an arterial phenotype.77 HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml−1 VEGF (Recombinant Human VEGF 165, Miltenyi Biotec) and 20 ng ml−1 bFGF (Human FGF-2, Miltenyi Biotec) in a T75 coated with 0.1% (w/v) gelatin (porcine skin type A, Sigma Aldrich) solution. .. A confluency of 80–90% was typically reached within 48–72 hours, after which hiPSC-ECs were disassociated using Tryple (Gibco).

    Immunofluorescence:

    Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19.
    Article Snippet: The expanded ECs were frozen in 40% EGM-2 (Promocell), 10% dimethyl sulfoxide and 50% fetal calf serum (v/v) to be thawed upon use. .. Differentiated endothelial cells have previously been extensively characterized using FACS, immunofluorescence and RNA sequencing, and have a distinct phenotype that mostly resembles an embryonic state, but also shows signs of an arterial phenotype.77 HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml−1 VEGF (Recombinant Human VEGF 165, Miltenyi Biotec) and 20 ng ml−1 bFGF (Human FGF-2, Miltenyi Biotec) in a T75 coated with 0.1% (w/v) gelatin (porcine skin type A, Sigma Aldrich) solution. .. A confluency of 80–90% was typically reached within 48–72 hours, after which hiPSC-ECs were disassociated using Tryple (Gibco).

    RNA Sequencing:

    Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19.
    Article Snippet: The expanded ECs were frozen in 40% EGM-2 (Promocell), 10% dimethyl sulfoxide and 50% fetal calf serum (v/v) to be thawed upon use. .. Differentiated endothelial cells have previously been extensively characterized using FACS, immunofluorescence and RNA sequencing, and have a distinct phenotype that mostly resembles an embryonic state, but also shows signs of an arterial phenotype.77 HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml−1 VEGF (Recombinant Human VEGF 165, Miltenyi Biotec) and 20 ng ml−1 bFGF (Human FGF-2, Miltenyi Biotec) in a T75 coated with 0.1% (w/v) gelatin (porcine skin type A, Sigma Aldrich) solution. .. A confluency of 80–90% was typically reached within 48–72 hours, after which hiPSC-ECs were disassociated using Tryple (Gibco).

    Clinical Proteomics:

    Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19.
    Article Snippet: The expanded ECs were frozen in 40% EGM-2 (Promocell), 10% dimethyl sulfoxide and 50% fetal calf serum (v/v) to be thawed upon use. .. Differentiated endothelial cells have previously been extensively characterized using FACS, immunofluorescence and RNA sequencing, and have a distinct phenotype that mostly resembles an embryonic state, but also shows signs of an arterial phenotype.77 HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml−1 VEGF (Recombinant Human VEGF 165, Miltenyi Biotec) and 20 ng ml−1 bFGF (Human FGF-2, Miltenyi Biotec) in a T75 coated with 0.1% (w/v) gelatin (porcine skin type A, Sigma Aldrich) solution. .. A confluency of 80–90% was typically reached within 48–72 hours, after which hiPSC-ECs were disassociated using Tryple (Gibco).

    Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19
    Article Snippet: .. HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml −1 VEGF (Recombinant Human VEGF 165, Miltenyi Biotec) and 20 ng ml −1 bFGF (Human FGF-2, Miltenyi Biotec) in a T75 coated with 0.1% (w/v) gelatin (porcine skin type A, Sigma Aldrich) solution. .. A confluency of 80–90% was typically reached within 48–72 hours, after which hiPSC-ECs were disassociated using Tryple (Gibco).

    Article Title: Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells.
    Article Snippet: Human induced pluripotent stem cells-derived endothelial cells generation and culture Endothelial cells were differentiated from hiPSCs throughmesoderm specification under defined culture conditions followed by cryopreservation until chip culture, as previously described.62 Cryopreserved hiPSC-derived endothelial cells were thawed in a water bath at 37 C and transferred into a 15 mL tube containing 7 mL of Human Endothelial SFM medium (11111044; Thermo Fisher Scientific). .. The cells were centrifuged at 300 x g for 3 min and the pellet was resuspended in Human Endothelial SFM complete medium: Human Endothelial SFMmedium supplemented with 20 ng/mL bFGF (130-093-564; Miltenyi Biotech), 30 ng/mL VEGF (130-109-396; Miltenyi Biotech), and 1% (v/v) human serum from platelet poor plasma (P2918; Sigma-Aldrich) and seeded into a T75 cell culture flask that was previously coated with 0.1% (w/v) gelatine solution for 1 h at 37 C. The cells were expanded for 3-4 days prior to seeding into the tumor-on-chip and used only at passage 1. .. Generation and culture of ROR1- and CD19-specific CAR-T cells PBMCs were isolated by density gradient centrifugation from leukocyte apheresis of two different healthy donors using a separating solution with a density of 1.077 g/mL (Pancoll human; PAN Biotech).

    Recombinant:

    Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19.
    Article Snippet: The expanded ECs were frozen in 40% EGM-2 (Promocell), 10% dimethyl sulfoxide and 50% fetal calf serum (v/v) to be thawed upon use. .. Differentiated endothelial cells have previously been extensively characterized using FACS, immunofluorescence and RNA sequencing, and have a distinct phenotype that mostly resembles an embryonic state, but also shows signs of an arterial phenotype.77 HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml−1 VEGF (Recombinant Human VEGF 165, Miltenyi Biotec) and 20 ng ml−1 bFGF (Human FGF-2, Miltenyi Biotec) in a T75 coated with 0.1% (w/v) gelatin (porcine skin type A, Sigma Aldrich) solution. .. A confluency of 80–90% was typically reached within 48–72 hours, after which hiPSC-ECs were disassociated using Tryple (Gibco).

    Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19
    Article Snippet: .. HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml −1 VEGF (Recombinant Human VEGF 165, Miltenyi Biotec) and 20 ng ml −1 bFGF (Human FGF-2, Miltenyi Biotec) in a T75 coated with 0.1% (w/v) gelatin (porcine skin type A, Sigma Aldrich) solution. .. A confluency of 80–90% was typically reached within 48–72 hours, after which hiPSC-ECs were disassociated using Tryple (Gibco).

    Cell Culture:

    Article Title: Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells.
    Article Snippet: Human induced pluripotent stem cells-derived endothelial cells generation and culture Endothelial cells were differentiated from hiPSCs throughmesoderm specification under defined culture conditions followed by cryopreservation until chip culture, as previously described.62 Cryopreserved hiPSC-derived endothelial cells were thawed in a water bath at 37 C and transferred into a 15 mL tube containing 7 mL of Human Endothelial SFM medium (11111044; Thermo Fisher Scientific). .. The cells were centrifuged at 300 x g for 3 min and the pellet was resuspended in Human Endothelial SFM complete medium: Human Endothelial SFMmedium supplemented with 20 ng/mL bFGF (130-093-564; Miltenyi Biotech), 30 ng/mL VEGF (130-109-396; Miltenyi Biotech), and 1% (v/v) human serum from platelet poor plasma (P2918; Sigma-Aldrich) and seeded into a T75 cell culture flask that was previously coated with 0.1% (w/v) gelatine solution for 1 h at 37 C. The cells were expanded for 3-4 days prior to seeding into the tumor-on-chip and used only at passage 1. .. Generation and culture of ROR1- and CD19-specific CAR-T cells PBMCs were isolated by density gradient centrifugation from leukocyte apheresis of two different healthy donors using a separating solution with a density of 1.077 g/mL (Pancoll human; PAN Biotech).

    other:

    Article Title: CTRP13-Mediated Effects on Endothelial Cell Function and Their Potential Role in Obesity
    Article Snippet: CTRP13 (Biorbyt, Cambridge, UK # orb155966), alpha-Tubulin (Cell Signaling, Danvers, MA, USA # 2144), GAPDH (Cell Signaling #2118), p53 (Cell Signaling #9282), phospho-p53 (Ser20; Cell Signaling # 9287), phospho-p53 (Ser15, Cell Signaling # 9284), p21 (Cell Signaling # 2947), phospho-Rb (Ser795, Cell Signaling # 9301), CD31 (AgilentDako, Santa Clara, CA, USA # M0823), p44/42 MAPK (Cell Signaling #9102), phospho-p44/42 MAPK (Cell Signaling #9101), AMPK (Cell Signaling #2532), phospho-AMPK (Thr172, Cell Signaling #2535), Akt (Cell Signaling #9272), phospho-Akt (Thr308, Cell Signaling #4056), V5 (ThermoFisher # R960-25), Smad2/3 (Cell Signaling # 5678), phospho-Smad2/3 (Cell Signaling # 8828), adenine 9-β-D-arabinofuranoside (Jena Bioscience, Jena Germany # NU-875), SB202190 (Sigma-Aldrich, Taufkirchen, Germany # 559388), U0126 (Sigma-Aldrich # U120), human TNF-alpha (Sigma-Aldrich # H8916), C87 (Bio-Techne, Wiesbaden, Germany # 5484), and VEGF (Miltenyi Biotec, Bergisch Gladbach, Germany # 130-127-426).



    Similar Products

    99
    NSJ Bioreagents vegfr2 antibody / vegf receptor 2 / kdr
    Vegfr2 Antibody / Vegf Receptor 2 / Kdr, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vegf/VEGFR2+Antibody+%2F+VEGF+Receptor+2+%2F+KDR/custom%40v8272%4042670667
    Average 99 stars, based on 1 article reviews
    vegfr2 antibody / vegf receptor 2 / kdr - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    94
    MedChemExpress recombinant human vegf a165
    Recombinant Human Vegf A165, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vegf/VEGF165%2C+Human/pmc13463935-118-5-9
    Average 94 stars, based on 1 article reviews
    recombinant human vegf a165 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Miltenyi Biotec vegf a
    Vegf A, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vegf/Human+VEGF+(165)+IS%2C+premium+grade/pmc13380759-111-13-17
    Average 94 stars, based on 1 article reviews
    vegf a - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc anti vegf a
    Anti Vegf A, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vegf/pmc12972984-281-32-33
    Average 86 stars, based on 1 article reviews
    anti vegf a - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    94
    Elabscience Biotechnology vegf
    Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, <t>VEGF,</t> HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.
    Vegf, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vegf/Rat+VEGF-A+(Vascular+Endothelial+Cell+Growth+Factor+A)+ELISA+Kit/pmc12992994-233-11-23
    Average 94 stars, based on 1 article reviews
    vegf - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

    Journal: Bioactive Materials

    Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration

    doi: 10.1016/j.bioactmat.2026.02.059

    Figure Lengend Snippet: Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

    Article Snippet: ELISA kits for PGE2 (Cat. No. E-EL-0034), TGF-β (Cat. No. E-EL-0162), VEGF (Cat. No. E-EL-R2603), and HGF (Cat. No. E-EL-R0496) were purchased from Elabscience (Wuhan, China).

    Techniques: Confocal Microscopy, Fluorescence, Staining, Cell Culture, Expressing