vegf a (Miltenyi Biotec)
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Vegf A, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vegf/Human+VEGF+(165)+IS%2C+premium+grade/pmc13380759-111-13-17
Average 94 stars, based on 26 article reviews
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FACS:Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19. Article Snippet: The expanded ECs were frozen in 40% EGM-2 (Promocell), 10% dimethyl sulfoxide and 50% fetal calf serum (v/v) to be thawed upon use. .. Differentiated endothelial cells have previously been extensively characterized using FACS, immunofluorescence and RNA sequencing, and have a distinct phenotype that mostly resembles an embryonic state, but also shows signs of an arterial phenotype.77 HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml−1 Immunofluorescence:Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19. Article Snippet: The expanded ECs were frozen in 40% EGM-2 (Promocell), 10% dimethyl sulfoxide and 50% fetal calf serum (v/v) to be thawed upon use. .. Differentiated endothelial cells have previously been extensively characterized using FACS, immunofluorescence and RNA sequencing, and have a distinct phenotype that mostly resembles an embryonic state, but also shows signs of an arterial phenotype.77 HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml−1 RNA Sequencing:Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19. Article Snippet: The expanded ECs were frozen in 40% EGM-2 (Promocell), 10% dimethyl sulfoxide and 50% fetal calf serum (v/v) to be thawed upon use. .. Differentiated endothelial cells have previously been extensively characterized using FACS, immunofluorescence and RNA sequencing, and have a distinct phenotype that mostly resembles an embryonic state, but also shows signs of an arterial phenotype.77 HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml−1 Clinical Proteomics:Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19. Article Snippet: The expanded ECs were frozen in 40% EGM-2 (Promocell), 10% dimethyl sulfoxide and 50% fetal calf serum (v/v) to be thawed upon use. .. Differentiated endothelial cells have previously been extensively characterized using FACS, immunofluorescence and RNA sequencing, and have a distinct phenotype that mostly resembles an embryonic state, but also shows signs of an arterial phenotype.77 HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml−1 Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19 Article Snippet: .. HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml −1 Article Title: Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells. Article Snippet: Human induced pluripotent stem cells-derived endothelial cells generation and culture Endothelial cells were differentiated from hiPSCs throughmesoderm specification under defined culture conditions followed by cryopreservation until chip culture, as previously described.62 Cryopreserved hiPSC-derived endothelial cells were thawed in a water bath at 37 C and transferred into a 15 mL tube containing 7 mL of Human Endothelial SFM medium (11111044; Thermo Fisher Scientific). .. The cells were centrifuged at 300 x g for 3 min and the pellet was resuspended in Human Endothelial SFM complete medium: Human Endothelial SFMmedium supplemented with 20 ng/mL bFGF (130-093-564; Miltenyi Biotech), 30 ng/mL Recombinant:Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19. Article Snippet: The expanded ECs were frozen in 40% EGM-2 (Promocell), 10% dimethyl sulfoxide and 50% fetal calf serum (v/v) to be thawed upon use. .. Differentiated endothelial cells have previously been extensively characterized using FACS, immunofluorescence and RNA sequencing, and have a distinct phenotype that mostly resembles an embryonic state, but also shows signs of an arterial phenotype.77 HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml−1 Article Title: Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19 Article Snippet: .. HiPSC-ECs were expanded human endothelial serum-free medium (EC-SFM, Gibco) supplemented with 1% platelet poor plasma (human, Sigma), 30 ng ml −1 Cell Culture:Article Title: Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells. Article Snippet: Human induced pluripotent stem cells-derived endothelial cells generation and culture Endothelial cells were differentiated from hiPSCs throughmesoderm specification under defined culture conditions followed by cryopreservation until chip culture, as previously described.62 Cryopreserved hiPSC-derived endothelial cells were thawed in a water bath at 37 C and transferred into a 15 mL tube containing 7 mL of Human Endothelial SFM medium (11111044; Thermo Fisher Scientific). .. The cells were centrifuged at 300 x g for 3 min and the pellet was resuspended in Human Endothelial SFM complete medium: Human Endothelial SFMmedium supplemented with 20 ng/mL bFGF (130-093-564; Miltenyi Biotech), 30 ng/mL other:Article Title: CTRP13-Mediated Effects on Endothelial Cell Function and Their Potential Role in Obesity Article Snippet: CTRP13 (Biorbyt, Cambridge, UK # orb155966), alpha-Tubulin (Cell Signaling, Danvers, MA, USA # 2144), GAPDH (Cell Signaling #2118), p53 (Cell Signaling #9282), phospho-p53 (Ser20; Cell Signaling # 9287), phospho-p53 (Ser15, Cell Signaling # 9284), p21 (Cell Signaling # 2947), phospho-Rb (Ser795, Cell Signaling # 9301), CD31 (AgilentDako, Santa Clara, CA, USA # M0823), p44/42 MAPK (Cell Signaling #9102), phospho-p44/42 MAPK (Cell Signaling #9101), AMPK (Cell Signaling #2532), phospho-AMPK (Thr172, Cell Signaling #2535), Akt (Cell Signaling #9272), phospho-Akt (Thr308, Cell Signaling #4056), V5 (ThermoFisher # R960-25), Smad2/3 (Cell Signaling # 5678), phospho-Smad2/3 (Cell Signaling # 8828), adenine 9-β-D-arabinofuranoside (Jena Bioscience, Jena Germany # NU-875), SB202190 (Sigma-Aldrich, Taufkirchen, Germany # 559388), U0126 (Sigma-Aldrich # U120), human TNF-alpha (Sigma-Aldrich # H8916), C87 (Bio-Techne, Wiesbaden, Germany # 5484), and |
